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Journal: medRxiv
Article Title: CLIA MDK: A Modular Smartphone Platform Matching Plate Reader Performance for Chemiluminescent Immunoassay Development
doi: 10.64898/2026.03.26.26348440
Figure Lengend Snippet: Mobile platform for the evaluation and development of CLIAs. a Workflow of the development and evaluation platform, including the selection of an image sensor, its acquisition conditions, measuring standard curves, and optimization studies. b Schematic illustration of the Mobile Camera Characterization System (MCCS) for the in silico sensor and signal processing evaluation. c Schematic illustration of the Mobile Reader (MR) for CLIA signal quantification. d Mobile app to control the MCCS and MR. e Quantification control and analysis for a biomarker standard curve targeting Renin. Analyte concentrations, the resulting raw image, and control of well detection accuracy (yellow circle). A squared region of interest (ROI, yellow box) and background (BG) indicate some of the areas assessed for signal and background quantification, respectively. f Box plot of raw pixel values from ROI as a function of the biomarker concentration analyzed by primary image channel (red, green, blue) with pairwise statistical significance test. The box represents the inter-quartile range (IQR), with the median (hereafter termed signal) shown as a horizontal line. Whiskers extend to the most extreme data points within 1.5 times the IQR. n = 3721 pixels.
Article Snippet: The
Techniques: Selection, In Silico, Control, Biomarker Discovery, Concentration Assay
Journal: medRxiv
Article Title: CLIA MDK: A Modular Smartphone Platform Matching Plate Reader Performance for Chemiluminescent Immunoassay Development
doi: 10.64898/2026.03.26.26348440
Figure Lengend Snippet: Camera Sensor Evaluation with MCCS. a Workflow using the MCCS to test configurations for CLIA testing. b Optical power limit for different cameras measured with a blue LED and varying numbers of neutral density filters (OD). Xiaomi 13 Pro (X13P), Huawei P60 Pro (HP60P), Huawei P20 (HP20), Fairphone 5 (F5), Nokia G22 (NG22), Arducam 3MP (AC3MP), Arducam 5MP (AC5MP), and OmniVision 5640 (OV5640). For reference, the cyan line is the power output of the signal from an aldosterone CLIA (sample conc. 100 pg mL −1 ). c Comparison of our (dark grey) and the smartphone’s native (light grey) app’s signal-to-noise ratio (SNR), signal, and noise ( n = 15 images). d Precision of signal quantification for signal (dark grey) and background (light grey) for the smartphones HP20 and X13P as the relative error (% of full-scale). e SNR as a function of image acquisition parameters using the X13P (ISO and exposure time (s)). f The effect of image processing algorithms on the SNR using the X13P with 17 OD 0.9 filters added above the LED, calculated for the raw signal (RAW, n = 225 average over 15 images for 15 sets), the ROF denoised signal (ROF, n = 225 average over 15 images for 15 sets), the NREA processed signal on RAW and ROF denoised images NREA ROF ( n = 15, each).
Article Snippet: The
Techniques: Comparison
Journal: medRxiv
Article Title: CLIA MDK: A Modular Smartphone Platform Matching Plate Reader Performance for Chemiluminescent Immunoassay Development
doi: 10.64898/2026.03.26.26348440
Figure Lengend Snippet: A method for rapid CLIA development and characterization using the MR device. a Schematic of the key steps and timings for the magnetic microparticle-based CLIA used in these studies. b 3D-rendering of custom-built magnetic microparticle transfer tool compatible with ANSI/SLAS Microplate Standard 96-well microplates or equivalent 8-well strips. c Focus control of our app, demonstrating out-of-focus and in-focus images using the MCCS. d Time-lapse capabilities of our app with fixed intervals in automatic mode (Automatic) or flexible interval defined by user (Manual) and resulting images acquired with MCCS. e Time dependence of signals for a renin (top) and aldosterone (bottom) standard curve with the start time of the signal reaching steady state (dashed line, on average 200 and 400 s for aldosterone and renin, respectively) and period during which the signal remains stable (grey box). f Intra-day (ID) and inter-day (InterD) measured on different days (D) with two technical replicates (R), assay signal and coefficient of variation (%CV) measured with the MR for five concentrations of a renin standard curve.
Article Snippet: The
Techniques: Control